Technical Assistant in T Cell Engineering and Molecular Biology (DE)

Employer

German Cancer Research Center, Heidelberg, Germany

Description

• Laboratory management, ordering consumables, onboarding new colleagues, coordinating repairs, annual inventory, etc.
• Molecular cloning, including site-directed mutagenesis of plasmids and Golden Gate assembly
• Generation and sequence verification of expression constructs; bacterial transformation, plasmid preparation, and maintenance of the plasmid and glycerol stock collection
• Maintenance of cell lines (e.g., Jurkat and derivatives)
• Transfection and electroporation of cell lines and primary cells; production and titration of lentiviral/retroviral vectors and transduction of target cells
• Isolation of PBMCs from whole blood and leukapheresis material; activation, expansion, and cryopreservation of primary human T cells, including rapid expansion protocols (REP)
• Documentation of work in accordance with genetic engineering (GenTG/S1–S2) and biosafety requirements
• Supervising students and apprentices

Stats

Post a comment

You need to be signed in to post comments. You can sign in here.

Comments

There are no comments yet.

More jobs

• 
  PhD Position on Automated Compliance Engineering University of Leuven
• 
  PhD Position - Data-Driven Development of Stable Wide-Bandgap Perovskite Subcells for Tandem Photovoltaics Julich Research Centre
• 
  Postdoctoral Researcher – Computational Electron Microscopy and Machine Learning Julich Research Centre
• 
  Koordinator Ledningsstöd KTH Royal Institute of Technology

See also

engineer jobs in Germany, engineer jobs in Heidelberg, all jobs